Metatranscriptome Sequencing

Community-wide transcriptome profiling to characterize microbial gene expression, functional activity, and responses across complex environments.

Metatranscriptomics utilizes environmental samples to analyze the complete set of transcripts at a given time, uncovering highly expressed genes within microbial ecosystems. Through next-generation sequencing (NGS)-based random RNA sequencing, it generates comprehensive expression profiles, captures temporal gene expression dynamics, and provides deeper insights into the structure, function, and adaptation of complex microbial communities.

Novogene employs advanced Illumina NovaSeq platforms for cDNA library preparation and sequencing, utilizing a paired-end 150-base-pair (150 bp) protocol to consistently deliver high-quality, high-fidelity short-read data. With extensive industry expertise and significant sequencing capacity, Novogene provides a comprehensive range of services designed to meet diverse research requirements seamlessly.

Benefits of Novogene Prokaryotic RNA Sequencing

Proven Expertise

Delivering over 1 million RNA-seq samples annually, ensuring reliability and scientific excellence.

Tailored Solutions

Receive customized workflows and analytical solutions designed specifically for complex or challenging samples.

Unmatched Data Quality

Achieve results that consistently surpass manufacturer performance benchmarks for accuracy and reliability.

Applications of Metatranscriptome Sequencing

The Metatranscriptome studies the entire transcriptional status and transcriptional regulatory patterns of the entire genome of a population at a specific time and in a specific environment. It can also conduct in situ research on the composition of active bacterial species in microbial communities and the expression of core genes.

Microbial community analysis

Transcriptional profiling of complex microbial community structure and traits.

Pathogen identification

Target pathogen identification for clinical and environmental monitoring.

Active microbes & functional potential

Exploration of dominant/active microbial species and their functional potentials.

Core gene screening & pathway analysis

Core gene screening & functional characterization with metabolic pathway analysis.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library Type	Sample Type	Amount	RNA Integrity Number	Purity
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0
Meta-transcriptome Library	Total RNA	≥ 1 μg	≥ 5.8, with flat baseline	A260/280 ≥ 2.0;  A260/230 ≥ 2.0

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina Sequencing System
Read Length	Paired-end 150bp
Recommended Data Amount	≥ 20 million read pairs per sample
Content of Data Analysis	• Data Quality Control  • Metatranscriptome Assembly  • Gene Functional Annotation  • rRNA& mRNA Taxonomic Analysis  • Gene expression quantification & Differential expression analysis  • Enrichment Analysis  • Functional comparison

Novogene Workflow of Metatranscriptome Sequencing Service

Our Metatranscriptome Sequencing workflow ensures high-quality results through a rigorous, step-by-step process:

Resources

1/1

Species-abundance clustering

This analysis clusters samples or species based on species abundance patterns to reveal similarities/differences in community composition and identify key taxa driving community structure variations.

1/1

Cluster analysis of samples based on species abundance

This analysis groups samples with similar species abundance patterns to reveal potential sample associations, ecological similarities, or grouping trends related to environmental factors or experimental treatments.

1/1

Phylogenetic tree of species differences

This tree visually illustrates evolutionary relationships and divergence patterns among species, aiding in identifying key differences in genetics, morphology, or ecology across taxa.

1/1

Circos plot of ARO abundance

This Circos plot intuitively visualizes the relative abundance and distribution patterns of Antimicrobial Resistance Genes (AROs) across samples or microbial taxa.

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Distribution- and abundance-based cluster analysis of resistance gene types

This analysis clusters resistance gene types based on their distribution patterns and abundance levels to reveal associations between gene groups and environmental/biological factors, supporting resistance mechanism research.

1/1

Volcano plot of differentially expressed genes

The Volcano plot of differentially expressed genes visually displays the fold change and statistical significance of gene expression differences between groups, enabling rapid identification of significantly upregulated/downregulated genes.

Resources

1/1

Species-abundance clustering

This analysis clusters samples or species based on species abundance patterns to reveal similarities/differences in community composition and identify key taxa driving community structure variations.

1/1

Cluster analysis of samples based on species abundance

1/1

Phylogenetic tree of species differences

This tree visually illustrates evolutionary relationships and divergence patterns among species, aiding in identifying key differences in genetics, morphology, or ecology across taxa.

1/1

Circos plot of ARO abundance

This Circos plot intuitively visualizes the relative abundance and distribution patterns of Antimicrobial Resistance Genes (AROs) across samples or microbial taxa.

1/1

Distribution- and abundance-based cluster analysis of resistance gene types

1/1

Volcano plot of differentially expressed genes

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