Whole Genome Bisulfite Sequencing (WGBS)

Genome-wide, single-base resolution profiling of DNA methylation to reveal epigenetic regulation and methylation patterns across the genome.

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling. Perturbations in methylation patterns are associated with tumorigenesis, neurodegenerative diseases and neurological disorders. Bioinformatic analysis of methylomes is widely used in various research areas, including studies on gene regulation, cell differentiation, embryogenesis, aging, occurrence and development of disease, phenotypic diversity and evolution in plants and animals.

Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.

Benefits

Profound Experience

Novogene has extensive experience in conducting WGBS on a wide variety of species including mammals, plants, fishes, insects, fungi, etc.

Low input and high efficiency

Ultra-low input DNA and high bisulfite conversion rate (>99%) are available in methylation library preparation.

Comprehensive Analysis

Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.

Customized solutions

Customized association analysis can explore correlations between methylation and gene expression.

Rapid and High Capacity

Our streamlined workflows and advanced sequencing platforms enable Superior turnaround efficiency.

Applications

Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:

Methylation profiling

Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution.

Differential methylation analysis

Identifying differentially methylated sites associated with experimental treatments or sample conditions.

Navigate molecular mechanism

Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles.

Diseases diagnosis

Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type	Required Amount	Purity
Genomic DNA	≥ 100 ng	1.8 < A260/280<2.0 no degradation, no contamination

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing Platform	Illumina NovaSeq X Plus
Read Length	Paired end 150 bp
Recommended sequencing depth	≥ 30× coverage for species with reference genome
Standard Data Analysis	· Data Quality Control · mCs detection, methylation level calculation · Methylation level and frequency distribution · Differentially Methylated Site (DMS) detection · Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation · Function enrichment of DMR-associated genes and DMP-associated genesVisualization of BS seq data · Comparative analysis (among samples)

Project Workflow

Resources

Demo Results

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Distribution of Genome Coverage

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Methylation Level Distribution on Whole Genome

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Heatmap Analysis for Methylation Levels of Gene Functional Region

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Methylation Level Distribution at Functional Genetic Elements

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Circos Plot for DMR Condition in three contexts (CG, CHG, CHH)

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Diagram of GO Enrichment

Webinars

Decoding Genome-Wide Methylation: A Comprehensive Guide to WGBS Technology

Unlock the full potential of Whole-Genome Bisulfite Sequencing (WGBS) — the gold standard for DNA methylation analysis. Join us to explore how this powerful technology can revolutionize your epigenetic research, from experimental design to biological insights.

Novogene’s Comprehensive Methylation Service WGBS, EM-seq, and mRNA-WGBS Analysis

This webinar will provide you with a comprehensive overview of whole genome methylation sequencing services at Novogene, including Whole Genome Bisulfite Sequencing (WGBS), Enzymatic methyl sequencing (EM-seq), and mRNA-WGBS associated analysis.

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