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Novogene
  • Novogene
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De Novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De Novo Sequencing

    Metagenomics

    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing

    Transcriptomics

    • mRNA Sequencing
    • Swift & Express mRNA Sequencing New!
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    • Total RNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Whole Transcriptome Sequencing

    Single Cell & Spatial Omics

    • 10x Single Cell Gene Expression
    • Illumina PIP-seq Single Cell 3’ RNA Sequencing New!
    • Spatial Transcriptomics Sequencing New!

    Epigenomics

    • Whole Genome Bisulfite Sequencing (WGBS)
    • Enzymatic Methylation Sequencing
    • Directed Methylation Sequencing (DM-Seq) New!
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • Cleavage Under Targets & Tagmentation (CUT&Tag) New!
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)
    • Reduced Representation Bisulfite Sequencing (RRBS)

    Proteomics

    • Quantitative Proteomics New!
    • PTM Proteomics New!
    • Olink Proteomics New!

    Metabolomics

    • Untargeted Metabolomics

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on Ultima Sequencer
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mRNA SequencingSwift & Express mRNA SequencingTotal RNA SequencingHuman Whole Genome SequencingWhole Exome Sequencing10x Single Cell Gene ExpressionIllumina PIP-seq Single Cell 3’ RNA SequencingSpatial Transcriptomics SequencingWhole Genome Bisulfite Sequencing (WGBS)Quantitative ProteomicsUntargeted MetabolomicsShotgun Metagenomics SequencingMetatranscriptome SequencingSequencing Only on Illumina SequencerSequencing Only on Ultima SequencerFull-Length Transcriptome SequencingChromatin Immunoprecipitation Sequencing (ChIP-seq)
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Novogene
  • Novogene
  • Genomics
    • Human Whole Genome Sequencing
    • Whole Exome Sequencing
    • Plant and Animal Whole Genome Sequencing
    • Plant and Animal De Novo Sequencing
    • Microbial Whole Genome Sequencing
    • Microbial De Novo Sequencing

    Metagenomics

    • Shotgun Metagenomics Sequencing
    • Amplicon Sequencing

    Transcriptomics

    • mRNA Sequencing
    • Swift & Express mRNA Sequencing New!
    • Full-Length Transcriptome Sequencing
    • Prokaryotic RNA Sequencing
    • Metatranscriptome Sequencing
    • Total RNA Sequencing
    • Small RNA Sequencing (sRNA‑seq)
    • Whole Transcriptome Sequencing

    Single Cell & Spatial Omics

    • 10x Single Cell Gene Expression
    • Illumina PIP-seq Single Cell 3’ RNA Sequencing New!
    • Spatial Transcriptomics Sequencing New!

    Epigenomics

    • Whole Genome Bisulfite Sequencing (WGBS)
    • Enzymatic Methylation Sequencing
    • Directed Methylation Sequencing (DM-Seq) New!
    • RNA Immunoprecipitation Sequencing (RIP-seq)
    • Chromatin Immunoprecipitation Sequencing (ChIP-seq)
    • Cleavage Under Targets & Tagmentation (CUT&Tag) New!
    • Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq)
    • Reduced Representation Bisulfite Sequencing (RRBS)

    Proteomics

    • Quantitative Proteomics New!
    • PTM Proteomics New!
    • Olink Proteomics New!

    Metabolomics

    • Untargeted Metabolomics

    Premade Library

    • Sequencing Only on Illumina Sequencer
    • Sequencing Only on Ultima Sequencer
  • PromotionsPromotions
    • Platforms
    • Service & Support
    • Automated Delivery Platform (Falcon)
    • Bioinformatics Analysis Tool (NovoMagic)
    • Customer Service System (CSS)
    • Case Study
    • Blog
    • Webinar
    • Brochure
    • Cancer Research
    • Immuno-oncology
    • Agrigenomics
    • Environment
    • Food Science
    • Human Microbiome
    • Plant and Animal Microbiome
    • Drug Discovery and Development
    • Rare and Complex Diseases
    • About Us
    • Our Locations
    • News & Events
    • Careers
  • Contact UsContact Us
    • mRNA Sequencing
    • Illumina Lane Sequencing

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CompanyCompany menu

Contact UsContact Us menu

Service SupportService Support menu

Services
mRNA SequencingSwift & Express mRNA SequencingTotal RNA SequencingHuman Whole Genome SequencingWhole Exome Sequencing10x Single Cell Gene ExpressionIllumina PIP-seq Single Cell 3’ RNA SequencingSpatial Transcriptomics SequencingWhole Genome Bisulfite Sequencing (WGBS)Quantitative ProteomicsUntargeted MetabolomicsShotgun Metagenomics SequencingMetatranscriptome SequencingSequencing Only on Illumina SequencerSequencing Only on Ultima SequencerFull-Length Transcriptome SequencingChromatin Immunoprecipitation Sequencing (ChIP-seq)
Company
About UsOur LocationsNews & EventsCareers
Contact Us
Contact Us
Service Support
Automated Delivery Platform (Falcon)Bioinformatics Analysis Tool (NovoMagic)Customer Service System (CSS)
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Copyright © 2026 Novogene Corporation Inc. All rights reserved. For Research Use Only.
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Full-Length Transcriptome Sequencing

Comprehensive full-length transcript sequencing to resolve transcript structures, isoform diversity, and gene expression complexity.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Full-length transcriptome sequencing provides end-to-end coverage of transcripts, enabling accurate isoform profiling, alternative splicing analysis, detection of fusion transcripts, and discovery of novel RNA species. Leveraging PacBio SMRT long-read sequencing, researchers can obtain high-confidence, assembly-free full-length cDNA reads for precise characterization of transcript diversity across medical, agricultural, and functional genomics studies. In parallel, Oxford Nanopore direct RNA Sequencing captures continuous, native RNA molecules without reverse transcription or PCR, preserving RNA modifications, poly(A) tail length, and structural information to reveal post-transcriptional regulation with exceptional depth.


With extensive long-read sequencing experience, advanced PacBio and Nanopore platforms, and rigorous quality standards, Novogene delivers highly accurate, reproducible, and comprehensive full-length transcriptome data.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Direct Profiling of Native RNA ModificationsDirect Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Direct Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Comprehensive & Reproducible Analytical WorkflowsComprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

Comprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

High-Throughput Capacity & Cost-Effective ServiceHigh-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

High-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Direct Profiling of Native RNA ModificationsDirect Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Direct Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Comprehensive & Reproducible Analytical WorkflowsComprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

Comprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

High-Throughput Capacity & Cost-Effective ServiceHigh-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

High-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

Applications of Full-Length transcriptome Sequencing in Research and Medicine

Discover the power of full-length transcriptome sequencing with Novogene's services, delivering unbiased and complete RNA profiles to unlock groundbreaking discoveries.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Applications of Full-Length transcriptome Sequencing in Research and Medicine

Discover the power of full-length transcriptome sequencing with Novogene's services, delivering unbiased and complete RNA profiles to unlock groundbreaking discoveries.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Demo Results

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Demo Results

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

More Services

10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)

More Services

10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
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Full-Length Transcriptome Sequencing

Comprehensive full-length transcript sequencing to resolve transcript structures, isoform diversity, and gene expression complexity.
OverviewOverview
BenefitsBenefits
ApplicationsApplications
SpecificationsSpecifications
ResourcesResources

Full-length transcriptome sequencing provides end-to-end coverage of transcripts, enabling accurate isoform profiling, alternative splicing analysis, detection of fusion transcripts, and discovery of novel RNA species. Leveraging PacBio SMRT long-read sequencing, researchers can obtain high-confidence, assembly-free full-length cDNA reads for precise characterization of transcript diversity across medical, agricultural, and functional genomics studies. In parallel, Oxford Nanopore direct RNA Sequencing captures continuous, native RNA molecules without reverse transcription or PCR, preserving RNA modifications, poly(A) tail length, and structural information to reveal post-transcriptional regulation with exceptional depth.


With extensive long-read sequencing experience, advanced PacBio and Nanopore platforms, and rigorous quality standards, Novogene delivers highly accurate, reproducible, and comprehensive full-length transcriptome data.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Direct Profiling of Native RNA ModificationsDirect Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Direct Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Comprehensive & Reproducible Analytical WorkflowsComprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

Comprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

High-Throughput Capacity & Cost-Effective ServiceHigh-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

High-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Direct Profiling of Native RNA ModificationsDirect Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Direct Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Comprehensive & Reproducible Analytical WorkflowsComprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

Comprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

High-Throughput Capacity & Cost-Effective ServiceHigh-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

High-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

Applications of Full-Length transcriptome Sequencing in Research and Medicine

Discover the power of full-length transcriptome sequencing with Novogene's services, delivering unbiased and complete RNA profiles to unlock groundbreaking discoveries.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Applications of Full-Length transcriptome Sequencing in Research and Medicine

Discover the power of full-length transcriptome sequencing with Novogene's services, delivering unbiased and complete RNA profiles to unlock groundbreaking discoveries.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline
OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.
10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene
• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Demo Results

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Demo Results

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

Image
Image
1/1
Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

Image
Image
1/1
Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

Image
Image
1/1
LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

Image
Image
1/1
Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

Image
Image
1/1
Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

More Services

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(10x Single Cell RNA Sequencing)
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Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)

More Services

10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
10x Single Cell RNA Sequencing
(10x Single Cell RNA Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Human Whole Genome Sequencing
(Human Whole Genome Sequencing)
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
Whole Genome Bisulfite Sequencing (WGBS)
(Whole Genome Bisulfite Sequencing (WGBS))
mRNA Sequencing
(mRNA Sequencing)
mRNA Sequencing
(mRNA Sequencing)
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