STOmics Spatial Gene Expression

Comprehensive spatial gene expression analysis to map transcriptomic landscapes across tissue sections and support the discovery of spatially defined cellular organization and function.

STOmics’ flagship technology, Spatial Enhanced REsolution Omics-sequencing (Stereo-seq), is an advanced spatial transcriptomics platform based on DNA Nano Ball (DNB) technology. This cutting-edge system offers unparalleled resolution, throughput, and field of view, enabling comprehensive spatial transcriptomic analysis across multiple scales—tissue, cellular, subcellular, and molecular levels. Using a patterned array chip and Coordinate ID (CID) system, Stereo-seq captures mRNA directly from tissue samples, restoring its precise spatial coordinates. As a transformative tool, Stereo-seq empowers researchers to elucidate the complex relationships between gene expression, cell morphology, and their local microenvironments.

Benefits of Novogene’s STOmics Spatial Transcriptomics Service

Large Area High Resolution

500 nm resolution with up to 13 cm × 13 cm capture area for gene and imaging analysis

Broad Sample Compatibility

Compatible with fresh-frozen and FFPE samples across diverse animal and plant tissue types

Pathology Integration

Single-section staining enables simultaneous pathological assessment and spatial transcriptomics analysis

Applications

Tumor Microenvironment Research

Characterize the molecular and cellular landscape of the tumor microenvironment.

Immunology

Map the spatial distribution and interactions of immune cells within tissues.

Neuroscience

Investigate neural cell function and expression patterns at unprecedented resolution.

Biomarker Discovery

Identify cell-specific biomarkers for disease diagnosis and therapy development.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Project Workflow

Resources

Demo Results

1/1

Space Ranger is used to count the UMI for each gene on every bin, obtaining a bin-gene expression matrix for the sample. After performing data QC, the figure below shows the distribution of UMI per gene across the spatial regions of the tissue.

1/1

Cluster_Spatial: spatial distribution map of clusters; Cluster_tSNE: tSNE two-dimensional spatial representation map of clusters; Cluster_UMAP: UMAP two-dimensional spatial representation map of clusters

1/1

The top 4 differentially expressed genes of each cluster are displayed in several ways. The figure presents the t-SNE and UMAP dimensional reduction visualization of differentially expressed genes.

Service flyers

Novogene Stereo-seq OMNI Spatial Transcriptome Sequencing

Stereo-seq is an advanced technology that combines transcriptomics with spatial information. It enables mapping gene expression in tissue samples while preserving their spatial organization. Stereo-seq OMNI, a revolutionary sequencing-based spatial transcriptomics solution specially designed for studying FFPE samples, provides high-definition spatial whole transcriptome information at single-cell scale.

More Services

Spatial Transcriptomics Sequencing

10x Single Cell RNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.

STOmics Spatial Gene Expression

Comprehensive spatial gene expression analysis to map transcriptomic landscapes across tissue sections and support the discovery of spatially defined cellular organization and function.

Benefits of Novogene’s STOmics Spatial Transcriptomics Service

Large Area High Resolution

500 nm resolution with up to 13 cm × 13 cm capture area for gene and imaging analysis

Broad Sample Compatibility

Compatible with fresh-frozen and FFPE samples across diverse animal and plant tissue types

Pathology Integration

Single-section staining enables simultaneous pathological assessment and spatial transcriptomics analysis

Applications

Tumor Microenvironment Research

Characterize the molecular and cellular landscape of the tumor microenvironment.

Immunology

Map the spatial distribution and interactions of immune cells within tissues.

Neuroscience

Investigate neural cell function and expression patterns at unprecedented resolution.

Biomarker Discovery

Identify cell-specific biomarkers for disease diagnosis and therapy development.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Project Workflow

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

*FFPE glass slides must be positive charged glass slides.

Service	Sample Type	Sample Amount	Preservation	Sample QC	Shipping
Stereo-seq OMNI (Animal with reference genome)	FFPE block (recommended)	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	Preservation After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
Stereo-seq OMNI (Animal with reference genome)	FFPE section	RNA quality control sample: 10-15 sections of 5 μm thick paraffin rolls (at least 10 sections for biopsy samples); Formal sample: 4-6 sections of 5 μm thick paraffin sections (adjacent sections).	Dry and sealed，storage time at 4°C< 30 days	DV200% ≥ 30%	Cold pack transportation

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Stereo-seq (FFPE)
Species	No species limitation
Resolution	500nm
Sequencing platform	BGI DNB-SEQ T7
Read length	PE75
Recommended Data Amount	2.5B reads per sample
Analysis	Data QC Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

Project Workflow

Resources

Demo Results

1/1

The top 4 differentially expressed genes of each cluster are displayed in several ways. The figure presents the t-SNE and UMAP dimensional reduction visualization of differentially expressed genes.

Service flyers

Novogene Stereo-seq OMNI Spatial Transcriptome Sequencing

More Services

Spatial Transcriptomics Sequencing

10x Single Cell RNA Sequencing

Ready to Start Your Project?

Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.